Q: How much DNA is required for the genotyping using RAPiD-Seq?
A: RAPiD-Seq requires 60 ng of high quality DNA. We ask for a minimum volume of 10 ┬Ál. Ideally, the DNA should be quantified in a fluorometer. We frequently observe an overestimation of the amount of DNA when quantified by spectrophotometer.
Q: What types of scientific studies are possible with RAPiD-Seq?
A: RAPiD-Seq is a flexible technology that allows the identification of a number of markers according to the objective of the study. The applications of this technology range from QTL mapping projects, to Marker-enabled selection (Genomic Selection) or Genome Wide Association Studies (GWAS).
Q: Can RAPiD-Seq be developed for my species?
A: Certainly. RAPiD-Seq technology can be developed and applied in potentially any species. For more information on the method development for your case, please contact support@rapid-genomics.com
Q: What type of primers do you use?
A: The structure of the primer used in the first PCR contains the sequence primer that is used by the sequencing platform, a degenerate sequence to confer stability to the reaction and the specific site, which determines the number of fragments to be amplified. The sequence of the specific site can be determined based on the diversity of the species and the number of the markers required by the client.
Q: How many fragments or tags does each primer generate?
A: The number of fragments generated by each primer depends on the sequence of the specific site and on the species being genotyped. RAPiD-Seq has the flexibility to identify the specific site that better fits the project and the species.
Q: How many fragments or tags does each primer generate?
A: Currently, RAPiD-Seq uses Illumina HiSeq. However, the method was designed in a way that when other platforms become available it can be easily converted.
Q: Can RAPiD-Seq be used if my species does not have a reference genome?
A: Yes, our analysis pipeline allows the identification of the markers based on the comparison of the individual reads to an haplotype bank that is created during the development process. For more information, please contact support@rapid-genomics.com
Q: How many genetic markers will RAPiD-Seq identify?
A: RAPiD-Seq technology is flexible regarding the number of markers identified. Based on the species diversity and the needs of the clients, we can choose a primer that better fits the project. The number of markers can potentially range from 1000 to 100,000 markers.